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Real-Time Measurements of Calcium and Contractility Parameters in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes (Video) | JoVE

Methods to Classify Cytoplasmic Foci as Mammalian Stress Granules

Journal (Biology)

Methods to Classify Cytoplasmic Foci as Mammalian Stress Granules

Isolation of Mouse Coronary Endothelial Cells

Journal (Biology)

Isolation of Mouse Coronary Endothelial Cells

Real-Time Measurements of Calcium and Contractility Parameters in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes (Video) | JoVE

Real-Time Measurements of Calcium and Contractility Parameters in hiPSC-CMs

Article DOI: 10.3791/65326-v • 01:21 min • May 26th, 2023

May 26th, 2023 •

Rafeeh Dinani^1,2, Emmy Manders^1,2,3, Michiel Helmes^1,2,3, Lili Wang⁴, Bjorn C. Knollmann⁴, Diederik W. D. Kuster^1,2, Jolanda van der Velden^1,2

¹Division of Physiology, Amsterdam UMC, Vrije University, ²Heart Failure & Arrhythmias, Amsterdam Cardiovascular Sciences, ³CytoCypher BV, ⁴Division of Clinical Pharmacology, Vanderbilt School of Medicine

Tags

Biology Contractility Parameters Human Induced Pluripotent Stem Cell-derived Cardiomyocytes Mutation-mediated Changes Stressors Drug Interventions Platform User-friendly Reproducible Contractility Calcium Handling Pixel Correlation Intracellular Calcium Transients Calcium-sensitive Fluorophore Temperature Environment Plate Formats Data Access Cellular Pathomechanisms Compounds Functionality Preclinical Drug Screening Process HiPSC-CMs

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