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Science Experiments

Basic Biology

Cell Counting with a Hemacytometer Grid

Monitoring Cell Line Health and Re-Seeding

PCR Steps and Reaction Setup

Running a DNA Gel Electrophoresis Test

Analyzing Protein Size by SDS-PAGE

Heat Shock for Plasmid DNA Uptake

Making Electrocompetent Bacterial Cells

Protein Detection with ELISA

Plasmid DNA Cleanup from Bacterial Cells

Purifying DNA From Agarose Gels

Protein Detection by Western Blot

How DNA and RNA Enter Mammalian Cells

DNA Ligase in Cloning and Repair

Restriction Enzymes: DNA Cutting Guide

Molecular Cloning in Bacteria

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Basic Methods in Cellular and Molecular Biology

Protein Detection with ELISA

Description

ELISA is a protein detection test that uses antibodies and a color change to identify a target in a sample. ELISA stands for enzyme-linked immunosorbent assay. It is called an immunoassay because antibodies do the detecting.

The test is carried out in a microtiter plate with small wells. In many ...

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Transcript

Das ELISA Verfahren, oder der Enzyme-linked Immunosorbent Assay, ist eine häufig verwendete Methode, um zu bestimmen ob ein bestimmtes Protein vorhanden ist oder nicht.

Mit einer Reihe von Wasch- und Bindungsschritten erkennt ein Antikörper, der an ein Enzym gekoppelt ist, ein Zielprotein auf der Oberfläche einer 96-Well...

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Tags

Enzyme linked Immunosorbent Assay Antik rperbindung Mikroplattenleser Blockierungspuffer Sandwich ELISA kompetitiver ELISA Prim rantik rper Sekund rantik rper kolorimetrischer Assay

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