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Basic Methods in Cellular and Molecular Biology
Making Electrocompetent Bacterial Cells
Description
Bacterial transformation can be done by making electrocompetent cells. In this process, foreign DNA enters bacterial cells after an electrical field creates small pores in the cell wall. The cells that can take up DNA from the environment are called competent cells. In the laboratory, scientists can produce electrocompetent ...
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Transcript
Bacterial transformation is a naturally occurring process, in which bacteria ingest foreign DNA and then amplify or clone it. In the lab, this process can be induced artificially, by using high voltage electric field pulses to create pores in the bacterial cell membrane, through which plasmid DNA can pass. Electroporation ...
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