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Basic Methods in Cellular and Molecular Biology
DNA Ligase in Cloning and Repair
Description
DNA ligase joins DNA fragments by forming a phosphodiester bond. The ligase enzyme carries out this reaction in cells and in the lab. It helps repair single-strand and double-strand breaks that can happen during DNA replication.
In molecular cloning, DNA ligase connects an insert with a vector, w...
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Transcript
Ligation can be defined as the act of joining, and in biology the term refers to an enzymatic reaction that joins two biomolecules with a covalent bond. This video describes the application of DNA ligation in molecular biology research.
In the cell, DNA ligases are enzymes that identify and s...
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