HIGH SCHOOL

Biology

Science Experiments

Basic Biology

Cell Counting with a Hemacytometer Grid

Monitoring Cell Line Health and Re-Seeding

PCR Steps and Reaction Setup

Running a DNA Gel Electrophoresis Test

Analyzing Protein Size by SDS-PAGE

Heat Shock for Plasmid DNA Uptake

Making Electrocompetent Bacterial Cells

Protein Detection with ELISA

Plasmid DNA Cleanup from Bacterial Cells

Purifying DNA From Agarose Gels

Protein Detection by Western Blot

How DNA and RNA Enter Mammalian Cells

DNA Ligase in Cloning and Repair

Restriction Enzymes: DNA Cutting Guide

Molecular Cloning in Bacteria

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Basic Methods in Cellular and Molecular Biology

Purifying DNA From Agarose Gels

Description

Purifying DNA from agarose gels lets students recover a separated DNA fragment after electrophoresis. The DNA is first isolated from the gel, then moved through a silica column for cleanup.

The process has three main steps: binding, washing, and eluting. DNA is believed to bind to silica when hig...

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Transcript

Gel-purification is a standard procedure performed to recover desired DNA fragments from agarose gels after electrophoretic separation. After dissolving the gel fragment and running it through a specialized filter, this procedure yields DNA freed from impurities such as salts, free nucleotides and enzymes, suitable for downs...

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Tags

DNA Recovery Silica Column Bind Wash Elute Agarose Gel Spin Column Ethanol Wash DNA Elution Gel Solubilization Downstream Applications

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