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Basic Methods in Cellular and Molecular Biology
Analyzing Protein Size by SDS-PAGE
Description
SDS-PAGE is a common lab method for analyzing proteins by size. It uses sodium dodecyl sulfate, or SDS, an anionic detergent that gives proteins a more even charge after they are linearized. The proteins are then loaded into a polyacrylamide gel and separated with an electric field.
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Transcript
SDS-PAGE is a technique used by many researchers to separate mixtures of proteins by size. Successful completion of this technique is an essential first step for many methods of protein analysis, like immunoblotting. By itself, it is a useful tool in assessing protein size and purity.
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