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Basic Methods in Cellular and Molecular Biology

Molecular Cloning in Bacteria

Description

Molecular cloning in bacteria uses a vector to carry recombinant DNA so it can replicate inside a host organism. The DNA fragment of interest, also called the insert, may come from a prokaryotic or eukaryotic specimen. A clear cloning strategy helps guide the work from the start.

The insert and t...

Transcript

Molecular cloning is a set of techniques used to insert recombinant DNA from a prokaryotic or eukaryotic source into a replicating vehicle such as plasmids or viral vectors. Cloning refers to making numerous copies of a DNA fragment of interest, such as a gene. In this video you will learn about the different steps of molecul...

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