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Basic Methods in Cellular and Molecular Biology
Heat Shock for Plasmid DNA Uptake
Description
Bacterial transformation lets cells take up foreign DNA from their surroundings. Some bacteria can do this naturally. In molecular biology, researchers recreate the process in the lab by making pores in the bacterial cell membrane.
Cells that can take up DNA from the environment are called compet...
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Transcript
Bacterial transformation is a widely used method where foreign DNA is introduced into a bacterium, which can then amplify, or clone the DNA. Cells that have the ability to readily take up this DNA are called competent cells. Although transformation is naturally occurring in many types of bacteria, scientists have found way...
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