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Basic Methods in Cellular and Molecular Biology
Running a DNA Gel Electrophoresis Test
Description
DNA gel electrophoresis separates DNA molecules using an electric field and an agarose gel matrix. The gel is made of agarose, which forms a porous network that helps DNA fragments move and sort by size. The negatively charged phosphates in the DNA backbone make the fragments migrate toward the anode, the positively charged ...
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Transcript
DNA Gel Electrophoresis is a technique used to separate and identify DNA fragments based on size.
DNA fragments of various sizes are loaded into a porous gel made from agarose – a carbohydrate found in red algae.
When an electric field is applied, the fragments will migrate through the gel, thanks to the negativ...
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