C Elegans Transgenic
C Elegans Transgenic - Related Videos
10:15
Generation of Transgenic C. elegans by Biolistic Transformation
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Cited by 27 •
2010
Transgenic worms are commonly used in C. elegans research. Described is a simple, yet effective, protocol to introduce transgenes into worms using biolistic bombardment with DNA-coated gold particles. The effort involved and results of bombardment compare favorably with microinjection for the generation of transgenic animals.
12:09
Generation of Stable Transgenic C. elegans Using Microinjection
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Cited by 93 •
2008
This video demonstrates the technique of microinjection into the gonad of C. elegans to create transgenic animals.
13:59
Assaying β-amyloid Toxicity using a Transgenic C. elegans Model
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Cited by 76 •
2010
The intensely studied nematode worm Caenorhabditis elegans can be transgenically engineered to express the human β-amyloid peptide (Aβ). Induced expression of Aβ in C. elegans muscle leads to a rapid, reproducible paralysis phenotype that can be used to monitor treatments that modulate Aβ toxicity.
12:03
The Production of C. elegans Transgenes via Recombineering with the galK Selectable Marker
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Cited by 4 •
2011
The ability to produce transgenes for Caenorhabditis elegans using genomic DNA carried by fosmids is particularly attractive as all of the native regulatory elements are retained. Described is a simple and robust procedure for the production of transgenes via recombineering with the galK selectable marker.
11:34
Basic Caenorhabditis elegans Methods: Synchronization and Observation
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Cited by 452 •
2012
The easiness of maintaining and propagating the nematode C. elegans make it a nice model organism to work with. The possibility of synchronizing worms allows the work with a significant amount of subjects at the same developmental stage, what facilitates the study of one particular process in many animals.
FAQs
The introduced DNA can persist in either of two heritable forms: an extrachromosomal array or a sequence integrated into the genome. These forms determine how the genetic material is maintained within a strain and how researchers interpret resulting gene expression or functional effects. This distinction is important when comparing stable transgenic lines and analyzing inherited biological changes.
Tissue-specific promoters act as regulatory sequences that direct a fluorescent reporter or functional gene to particular tissues. This targeting allows researchers to associate observed signals or biological effects with defined cellular locations rather than the whole animal. As a result, transgenic experiments can examine localized processes during development, signaling, behavior, or disease-related pathway studies.
Microinjection into the gonad places introduced DNA near the germline, the cell lineage that produces offspring. DNA entering this lineage can be passed to subsequent generations, allowing researchers to maintain and study transgenic strains over time. Heritability makes it possible to compare consistent genetic backgrounds and examine how altered gene activity affects the organism across its life cycle.
Researchers can introduce either a fluorescent reporter to visualize gene-related activity or a functional gene to test its biological effects. Observing fluorescence or changes in the animal provides a way to relate altered genetic activity to processes such as development, behavior, and signaling. This living-organism context helps connect molecular changes with phenotypic outcomes.
A typical workflow begins by preparing foreign or modified DNA, selecting regulatory sequences when tissue-specific expression is needed, and microinjecting the DNA into the gonad. The introduced material may then form an extrachromosomal array or integrate into the genome. Researchers can subsequently examine inherited expression or functional effects in the resulting strain.
Fluorescent reporters are useful when the goal is to observe cellular processes in real time. With an appropriate tissue-specific promoter, fluorescence can reveal where a gene-related signal or activity occurs within the animal. This approach supports studies of development and signaling and provides visual evidence that complements analyses of behavior or other biological outcomes.
Its short life cycle and well-characterized genetics make C. elegans an efficient system for producing and analyzing transgenic strains. Researchers can use these advantages to investigate gene function in a living organism while tracking effects on development, behavior, signaling, or disease-related pathways. The model also supports rapid observation of inherited genetic changes and cellular processes.