Seahorse Xf Analyzer
Seahorse Xf Analyzer - Related Videos
07:20
Bioenergetic Profile Experiment using C2C12 Myoblast Cells
0 Views •
Cited by 190 •
2010
A description of a method for profiling mitochondrial function in cells is provided. The mitochondrial profile generated provides four parameters of mitochondrial function that can be measured in one experiment: basal respiration rate, ATP-linked respiration, proton leak, and reserve capacity.
05:41
Metabolic Profile Analysis of Zebrafish Embryos
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Cited by 42 •
2013
Zebrafish represent a powerful vertebrate model that has been under-utilised for metabolic studies. Here we describe a rapid way to measure the in vivo metabolic profile of developing zebrafish that allows the comparison of different mitochondrial function parameters between genetically or pharmacologically manipulated embryos, thereby increasing the applicability of this organism.
11:27
Preparation and Respirometric Assessment of Mitochondria Isolated from Skeletal Muscle Tissue Obtained by Percutaneous Needle Biopsy
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Cited by 24 •
2015
Methods for biopsy of Vastus lateralis, preparation of purified mitochondria, and respirometric profiling are described. The use of small muscle volume makes this technique suitable for clinical research applications.
10:39
Using Isolated Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High throughput Microplate Respiratory Measurements
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Cited by 51 •
2015
The methods presented provide step-by-step instructions for the performance of a collection of microplate based respirometric assays using isolated mitochondria from minimal quantities of mouse skeletal muscle. These assays are able to measure mechanistic changes/adaptations in mitochondrial oxygen consumption in a commonly used animal model.
11:40
Bioenergetics and the Oxidative Burst: Protocols for the Isolation and Evaluation of Human Leukocytes and Platelets
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Cited by 74 •
2014
Blood leukocytes and platelets can be used as a marker of overall bioenergetic health of an individual and so have the potential to monitor pathological processes and the impact of treatments. Here we describe a method to isolate and measure mitochondrial function and the oxidative burst in these cells.
FAQs
OCR provides a readout of mitochondrial respiration, whereas ECAR indicates glycolytic activity through extracellular acidification. Examining both measurements helps researchers determine whether cells rely more heavily on oxidative or glycolytic energy production. Their combined patterns can reveal changes in cellular energy use during metabolic studies rather than describing only one pathway in isolation.
Substrates, metabolic inhibitors, or other compounds can be introduced during an assay to challenge cellular metabolism while measurements continue. The resulting changes in oxygen consumption or extracellular acidification show how cells respond to pathway-specific manipulation. This approach helps separate mitochondrial and glycolytic contributions and evaluates whether a compound alters cellular energy production.
Live-cell analysis captures metabolic activity while cells remain in their experimental state, allowing energy-use changes to be followed in real time. The sensor cartridge detects oxygen and acidification changes in small-volume wells, making it possible to monitor cellular flux during compound exposure. This temporal information supports analysis of dynamic responses rather than a single endpoint.
Researchers can inject metabolic inhibitors, substrates, or other compounds during the assay. These additions provide controlled perturbations that test how cells produce and use energy under altered conditions. Comparing measurements before and after an injection can reveal pathway dependence, treatment responses, or changes associated with cellular stress, provided the selected treatment addresses the biological question.
The system supports investigations of mitochondrial function, metabolic reprogramming, drug effects, disease mechanisms, and cellular stress. Researchers can use changes in OCR and ECAR to examine how energy metabolism shifts across these conditions. The resulting flux profiles connect cellular behavior with alterations in respiration or glycolytic activity, helping characterize metabolic phenotypes.
Disease-related changes or cellular stress can alter the balance between mitochondrial respiration and glycolytic activity. By measuring OCR and ECAR while applying relevant compounds, researchers can characterize those metabolic responses in living cells. This makes the platform useful for linking altered energy production with disease mechanisms, stress responses, and potential effects of metabolic treatments.