Tissue Cassette Preparation
Tissue Cassette Preparation - Related Videos
11:03
Pouring and Running a Protein Gel by reusing Commercial Cassettes
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Cited by 2 •
2012
Our protocol demonstrates how to pour multiple protein gels at a time by recycling Invitrogen Nupage Novex minigel cassettes, and inexpensive materials purchased at a home improvement store. This economical and streamlined method includes a way to store the gels at 4°C for a few weeks. By re-using the plastic gel cassettes from commercially available gels, labs that run frequent protein gels can save significant costs and help the environment.
09:37
Screening Foodstuffs for Class 1 Integrons and Gene Cassettes
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Cited by 13 •
2015
This protocol describes the detection of class 1 integrons and their associated gene cassettes in foodstuffs.
07:42
Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses
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Cited by 166 •
2016
Accurate identification and location of epithelial cells along the intestinal mucosal lining are essential to define different cell lineages. Proper imaging of intestinal tissues is crucial for identification of protein expression patterns with maximum resolution. This study aims to delineate the optimal methods and conditions for processing mouse intestinal tissues.
08:21
Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)
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Cited by 11 •
2012
A quick and efficient method to integrate foreign DNA of interest into pre-made acceptor strains, termed landing pad strains, is described. The method allows site-specific integration of a DNA cassette into the engineered landing pad locus of a given strain, through conjugation and expression of the ΦC31 integrase.
08:47
Preparation of Mouse Brain Tissue for Immunoelectron Microscopy
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Cited by 52 •
2010
We describe a protocol for transcardiac perfusion of mice, removal and sectioning of the brain, as well as immunoperoxidase staining, resin embedding, and ultrathin sectioning of the brain sections. Upon completion of these procedures, the immunostained material is ready for examination with transmission electron microscopy.
FAQs
Positioning the specimen so the region of interest is preserved determines which tissue surfaces and structures remain available for later examination. Appropriate trimming and orientation help retain relevant architecture during fixation, processing, embedding, and sectioning. This is especially important when inflammatory changes or pathogen-associated alterations are localized rather than distributed uniformly throughout the tissue.
Cassette permeability allows fixatives and subsequent processing reagents to contact the tissue rather than remaining limited to its outer surface. This supports the sequence of dehydration, clearing, and paraffin infiltration needed before embedding and sectioning. Effective reagent access helps maintain a specimen that can be examined microscopically with greater consistency.
Careful preparation helps preserve tissue architecture and pathogen-associated changes through the stages leading to microscopy. Because histopathology and immunohistochemistry depend on the retained structure and condition of the specimen, trimming, orientation, and secure enclosure can affect how inflammatory or immune responses are evaluated in the resulting sections.
After collection, the tissue is trimmed to an appropriate size, positioned to preserve the region of interest, and secured in a labeled cassette. The enclosed specimen then undergoes fixation and processing, including dehydration, clearing, and paraffin infiltration. These stages prepare the tissue for embedding and later sectioning for microscopic examination.
This preparation is used when tissue must be preserved for histopathology, immunohistochemistry, or assessment of inflammatory and immune responses. It provides a controlled route from collected tissue to microscopic sections, allowing investigators to examine architecture alongside pathogen-associated changes. The approach therefore supports studies that connect tissue structure with infection-related or immune-mediated findings.
Properly prepared tissue can retain structural features needed to evaluate tissue architecture, pathogen-associated changes, and patterns of inflammation or immune response. Histopathology supports examination of overall microscopic changes, while immunohistochemistry can be used to assess relevant features within the preserved specimen. The quality of cassette preparation helps determine how reliably these findings can be interpreted.