Fitc Nanoparticles
Fitc Nanoparticles - Related Videos
01:58
Studying the Permeation of FITC and FITC-Ferritin through an In Vitro Blood-Brain Barrier Model
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2025
This video demonstrates a method to study permeation through an in vitro blood-brain barrier (BBB) model using astrocytes and endothelial cells on a membrane insert, with free FITC and FITC-loaded ferritin. FITC-ferritin bound to receptors on endothelial cells and crossed the membrane barrier to the lower chamber while free FITC showed restricted permeation and remained in the upper chamber.
02:56
FITC-Dextran Feeding: A Method to Quantify Intestinal Permeability in C. elegans
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2023
This video introduces a method of to visualize and measure the integrity of the C. elegans intestine lumen by feeding worms FITC-labeled dextran. The example protocol shows the assay done with 3,3’-diindolylmethane (DIM)-treated and pathogen-fed worms.
04:50
Imaging FITC-dextran as a Reporter for Regulated Exocytosis
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Cited by 10 •
2018
Here we detail a method for live cell imaging of regulated exocytosis. This method utilizes FITC-dextran, which accumulates in lysosome-related organelles, as a reporter. This simple method also allows distinguishing between different modes of regulated exocytosis in cells that are difficult to manipulate genetically.
11:13
In Vitro Permeation of FITC-loaded Ferritins Across a Rat Blood-brain Barrier: a Model to Study the Delivery of Nanoformulated Molecules
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Cited by 15 •
2016
A method to establish an in vitro model of blood-brain barrier based on a co-culture of rat brain microvascular endothelial cells and astrocytes is described and validated. This system proved to be a valid tool to study the effect of nanoformulation on the trans-barrier permeation of fluorescent molecules.
09:23
Harmonic Nanoparticles for Regenerative Research
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Cited by 1 •
2014
Protocol details are provided for in vitro labeling human embryonic stem cells with second harmonic generating nanoparticles. Methodologies for hESC investigation by multi-photon microscopy and their differentiation into cardiac clusters are also presented.
FAQs
FITC reacts with primary amine groups located on nanoparticle surfaces or on biomolecules attached to the particles. This reaction forms a stable thiourea linkage, connecting the fluorescent label to the nanoparticle-associated material. The chemical attachment allows researchers to observe particle-associated signals while investigating how the labeled system behaves in biological environments.
The thiourea linkage provides a stable connection between FITC and the amine-containing nanoparticle surface or biomolecule. Stability helps preserve the association between fluorescence and the material being studied during biological observations. Consequently, detected green fluorescence can support analyses of nanoparticle distribution, cellular uptake, tissue localization, and transport in medicine-related experiments.
Fluorescence microscopy can show where labeled nanoparticles appear within cells or tissues, providing spatial information about localization and distribution. Flow cytometry measures fluorescent signals from cells, supporting analysis of cellular uptake across a population. Using these complementary approaches helps researchers connect nanoparticle-associated fluorescence with biological transport and cellular behavior.
A study first uses FITC labeling through reactions with primary amine groups on the nanoparticle surface or attached biomolecules. The labeled particles are then examined in biological systems with suitable excitation light. Fluorescence microscopy or flow cytometry provides the readout, allowing investigators to assess uptake, localization, distribution, or transport without relying on an unlabeled visual signal.
In nanoparticle-based drug delivery research, fluorescence tracking shows whether particles reach the cells or tissues relevant to a targeting strategy. Researchers can compare the observed distribution and localization with the intended delivery pattern. These observations help assess how nanoparticle behavior relates to targeting performance and support evaluation of emerging nanomedicine designs.
FITC-based tracking links nanoparticle behavior with biological responses, including cellular uptake, tissue localization, and transport. This connection supports broader assessments of biodistribution, targeting, safety, and effectiveness for nanoparticle-based drug delivery systems. The approach therefore provides a way to study both where particles go and how their distribution relates to evaluating a nanomedicine.