Lung Biopsy
Lung Biopsy - Related Videos
08:49
Exosomal miRNA Analysis in Non-small Cell Lung Cancer (NSCLC) Patients' Plasma Through qPCR: A Feasible Liquid Biopsy Tool
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Cited by 59 •
2016
This protocol describes the feasibility to perform miRNA profiling in exosomes, released in plasma of NSCLC patients, through a commercial exosome isolation kit with Proteinase K and RNAse treatments, in order to avoid circulating miRNAs contamination and evaluate their biomarker features in NSCLC.
11:02
Human Lung Dendritic Cells: Spatial Distribution and Phenotypic Identification in Endobronchial Biopsies Using Immunohistochemistry and Flow Cytometry
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Cited by 11 •
2017
Lung-resident immune cells, including dendritic cells (DCs) in humans, are critical for defense against inhaled pathogens and allergens. However, due to the scarcity of human lung tissue, studies are limited. This work presents protocols to process human mucosal endobronchial biopsies for studying lung DCs using immunohistochemistry and flow cytometry.
07:06
A Simple Method of Mouse Lung Intubation
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Cited by 50 •
2013
This paper describes a striaghforward and efficient method of intubating mice for pulmonary function measurements or pulmonary instillation, that allows the mice to recover and be studied at later times. The procedure involves an inexpensive fiberoptic light source that directly illuminates the trachea.
05:26
A Standardized Liquid Biopsy Preanalytical Protocol for Downstream Circulating-Free DNA Applications
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Cited by 3 •
2022
The liquid biopsy has revolutionized our approach to oncology translational studies, with sample collection, quality, and storage being crucial steps for its successful clinical application. Here we describe a standardized and validated protocol for downstream circulating-free DNA applications that can be applied in most translational research laboratories.
01:47
Lung Capacity
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2019
The air in the lungs is measured in volumes and capacities. Lung volume measures reflect the amount of air taken in, released, or left over after a lung function, like a single inhalation. Lung capacity measures are sums of two or more lung volume measures. There are four defined lung volume measures: tidal volume, inspiratory reserve volume, expiratory reserve volume, and residual volume. Tidal volume is the amount of air inhaled and exhaled in a normal breath. Inspiratory...
FAQs
Method selection depends largely on where the abnormality lies and on characteristics of the patient. A lesion’s location may favor bronchoscopy, a needle passed through the chest wall, or a surgical approach. Clinicians also weigh the expected diagnostic yield against procedure-related risks, including bleeding and pneumothorax, rather than using one technique for every case.
Bronchoscopy obtains tissue through the airways with biopsy instruments, while needle biopsy accesses a target through the chest wall. Minimally invasive or open surgery provides another route when those approaches are not appropriate or sufficient. These options differ in access and invasiveness, so clinicians match the approach to lesion location and patient factors.
Microscopic examination allows pathology to evaluate the tissue’s disease pattern, while molecular examination adds information at the molecular level. Together, these assessments can help distinguish cancer from infection, inflammation, and fibrotic disease. In suspected malignancy, the findings can also support tumor classification, giving clinicians more specific diagnostic information than imaging alone.
A biopsy must provide tissue that is sufficiently informative while exposing the patient to as little procedural harm as possible. A method with inadequate yield may fail to clarify the abnormality, whereas a more invasive approach may increase complications such as bleeding or pneumothorax. This balance makes risk and expected diagnostic value central to clinical planning.
Clinicians first select an access route according to the lesion’s location and the patient’s circumstances. Tissue is then obtained through bronchoscopy, chest-wall needle access, or surgery, depending on that decision. The specimen proceeds to pathology for microscopic and molecular examination, and the resulting findings guide disease identification, tumor classification, or treatment planning.
Lung biopsy is particularly useful when abnormal imaging findings or respiratory symptoms could reflect several different disease processes. Tissue-based evaluation can separate cancer from infection, inflammation, and fibrotic disease, which may appear clinically similar. For cancer, the specimen can further classify the tumor and provide information relevant to selecting a treatment plan.