Human Lung Dendritic Cells: Spatial Distribution and Phenotypic Identification in Endobronchial Biopsies Using Immunohistochemistry and Flow Cytometry

Faezzah Baharom; Gregory Rankin; Saskia Scholz; Jamshid Pourazar; Clas Ahlm; Anders Blomberg; Anna Smed-S&#246;rensen

doi:10.3791/55222

Human Lung Dendritic Cells: Spatial Distribution and Phenotypic Identification in Endobronchial B...

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Immunology and Infection

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JoVE Journal Immunology and Infection

Human Lung Dendritic Cells: Spatial Distribution and Phenotypic Identification in Endobronchial Biopsies Using Immunohistochemistry and Flow Cytometry

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11:02 min

January 20, 2017

DOI: 10.3791/55222-v

Faezzah Baharom¹, Gregory Rankin², Saskia Scholz¹, Jamshid Pourazar², Clas Ahlm³, Anders Blomberg², Anna Smed-Sörensen¹

¹Immunology and Allergy Unit, Department of Medicine Solna,Karolinska Institutet, ²Division of Medicine, Department of Public Health and Clinical Medicine,Umeå University, ³Divison of Infectious Diseases, Department of Clinical Microbiology,Umeå University

Lung-resident immune cells, including dendritic cells (DCs) in humans, are critical for defense against inhaled pathogens and allergens. However, due to the scarcity of human lung tissue, studies are limited. This work presents protocols to process human mucosal endobronchial biopsies for studying lung DCs using immunohistochemistry and flow cytometry.

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