Adult Mouse Brain
Adult Mouse Brain - Related Videos
03:48
Isolation of Microglia from the Cortex of an Adult Mouse Brain
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2025
The video demonstrates a technique for isolating microglia from the cortex of an adult mouse brain. First, the cortex undergoes segmentation and homogenization to remove neurons and other glial cells, preserving microglia. Following that, the cell suspension is exposed to anti-myelin magnetic microbeads and a magnetic field, effectively separating microglia and myeloid cells from myelin debris and cells containing myelin.
09:27
Whole Cell Recordings from Brain of Adult Drosophila
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Cited by 40 •
2007
This video demonstrates the procedure for isolating whole brains from adult Drosophila in preparation for recording from single neurons using standard whole cell technology. It includes images of GFP labeled cells and neurons viewed during recording.
09:42
Electrophysiological Recording in the Brain of Intact Adult Zebrafish
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Cited by 14 •
2013
This paper describes how an adult zebrafish can be immobilized, intubated, and used for in vivo electrophysiological experiments to allow recordings and manipulation of neural activity in an intact animal.
11:01
Isolation and Culture of Adult Zebrafish Brain-derived Neurospheres
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Cited by 28 •
2016
Here we provide a reproducible method to examine adult neurogenesis using a neurosphere assay derived from the whole brain or from either the telencephalic, tectal or cerebellar regions of the adult zebrafish brain. Additionally, we describe the procedure to manipulate gene expression in zebrafish neurospheres.
06:42
Cerebrovascular Casting of the Adult Mouse for 3D Imaging and Morphological Analysis
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Cited by 29 •
2011
In this article, we present a simple, practical technique for cerebrovascular casting that is easy to perform and can be utilized to image the vascular tree of the adult mouse brain.
FAQs
Neural communication combines electrical activity within neurons with neurotransmitter release at synapses, the specialized junctions where cells exchange signals. This sequence allows activity to move through interconnected circuits rather than remaining confined to individual cells. Studying these processes helps neuroscientists relate cellular signaling to functions such as sensation, movement, learning, and behavior.
Glial cells regulate the extracellular environment surrounding neurons, contribute to metabolic support, and participate in tissue responses. These functions influence the conditions under which neurons communicate and circuits operate. Examining glial activity therefore adds information that cannot be obtained from neuronal electrical signals alone, particularly in studies of brain injury, aging, and neurological disease.
Interconnected regions allow researchers to examine how distributed neural circuits generate coordinated functions. A circuit perspective connects activity in particular areas with broader outcomes, including sensation, movement, learning, and behavior. Well-characterized anatomy helps investigators map these relationships and interpret cellular or physiological findings within the larger organization of the brain.
Its mature neural circuits provide a framework for examining brain function under conditions such as aging, injury, or neurological disease. Researchers can compare cellular mechanisms, circuit organization, and observed functions across these contexts. This approach helps identify how changes at the tissue or cellular level relate to altered neurophysiology and behavior.
Researchers can combine anatomical analysis with neurophysiological measurements, imaging, and behavioral methods. Anatomy describes regional organization, neurophysiology examines neural activity, imaging supports observation of brain processes, and behavior connects those findings to functional outcomes. Using complementary approaches helps bridge different levels of analysis instead of relying on a single measurement.
The model combines genetic accessibility with well-characterized anatomy and compatibility with imaging and behavioral methods. These features make it practical to connect cellular mechanisms with neural-circuit function and observable behavior. Its established use across studies of neurophysiology, aging, injury, and disease also supports investigations that span multiple areas of neuroscience.
Studies can relate neuronal communication, glial regulation, and circuit organization to measurable functions such as sensation, movement, learning, and behavior. They can also provide context for evaluating potential treatments in neurological disease research. The value lies in connecting cellular observations with system-level outcomes rather than interpreting either level in isolation.