Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Developmental Biology / Isolation and Culture of Adult Zebrafish…
JoVE Journal
Developmental Biology

A subscription to JoVE is required to view this content.

JoVE Journal Developmental Biology
Isolation and Culture of Adult Zebrafish Brain-derived Neurospheres
Methods for Imaging Intracellular pH of the Follicle Stem Cell Lineage in Live Drosophila Ovarian Tissue
Journal (Developmental Biology)
Methods for Imaging Intracellular pH of the Follicle Stem Cell Lineage in Live Drosophila Ovarian Tissue
Live Cell Imaging of Microtubule Cytoskeleton and Micromechanical Manipulation of the Arabidopsis Shoot Apical Meristem
Journal (Developmental Biology)
Live Cell Imaging of Microtubule Cytoskeleton and Micromechanical Manipulation of the Arabidopsis Shoot Apical Meristem
 

Isolation and Culture of Adult Zebrafish Brain-derived Neurospheres

Article DOI: 10.3791/53617-v • 11:01 min • February 29th, 2016
February 29th, 2016 •
*1,2, *3, 1, 1,3, 1
1Yale Cardiovascular Research Center, Internal Medicine, Yale University, 2Department of Medicine, University of California, San Diego, 3APHP Groupe Hospitalier Pitié-Salpètrière, Université Pierre and Marie Curie
* These authors contributed equally

Chapters

Summary
Automatic Translation

Here we provide a reproducible method to examine adult neurogenesis using a neurosphere assay derived from the whole brain or from either the telencephalic, tectal or cerebellar regions of the adult zebrafish brain. Additionally, we describe the procedure to manipulate gene expression in zebrafish neurospheres.

Tags

Isolation Culture Adult Zebrafish Neurospheres Neurogenesis Gene Expression Neural Stem Cells Self-renewal Determination Technique Simple Reliable In Vitro Epigenetic Regulation Mouse Neurosphere Protocol Gel Packs Petri Dish Filter Paper Plastic Film Micro-dissection Instruments Sterilized Tools Dissecting Microscope
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter