Micro Pipette Harvesting
Micro Pipette Harvesting - Related Videos
05:30
Making Patch-pipettes and Sharp Electrodes with a Programmable Puller
0 Views •
Cited by 22 •
2008
This video shows how to use a programmable puller to make patch pipettes and sharp electrodes for electrophysiology. The same procedure can be used to make a variety of glass tools, including injection needles.
06:35
Introduction to Serological Pipettes and Pipettors
0 Views •
2023
The serological pipette is frequently used in the laboratory for transferring milliliter volumes of liquid, from less than 1 ml to up to 50 ml. The pipettes can be sterile, plastic, and disposable or sterilizable, glass and reusable. Both kinds of pipettes use a pipet-aid, for the aspiration and dispensation of liquids. Different sizes of pipettes can be used with the same pipet-aid for a variety of experimental assays. For example, serological pipettes are useful for mixing chemical solutions...
10:54
In Vivo Three-Dimensional Two-Photon Microscopy to Study Conducted Vascular Responses by Local ATP Ejection Using a Glass Micro-Pipette
0 Views •
Cited by 10 •
2019
We present an optimized local ejection procedure using a glass micro-pipette and a fast two-photon hyperstack imaging method, which allows precise measurement of capillary diameter changes and investigation of its regulation in three dimensions.
05:12
Pressure-polishing Pipettes for Improved Patch-clamp Recording
0 Views •
Cited by 13 •
2008
This is a guide to modifying the shape of glass micropipettes. Specifically, by using heat and air pressure the taper is widened without increasing the tip opening, leading to lower pipette resistance. This is critical to obtain low noise recordings of small cells but is useful in many applications.
11:43
Aseptic Laboratory Techniques: Volume Transfers with Serological Pipettes and Micropipettors
0 Views •
Cited by 24 •
2012
When working in a laboratory, it is imperative to minimize sources of contamination. Aseptic technique refers to procedures that permit transfer of cultures and reagents while avoiding contact with non-sterile surfaces. Serological pipettes and micropipettors are used to measure precise volumes without compromising sterility of solutions used in experiments.
FAQs
Controlled negative pressure draws the selected cell or tissue into the pipette rather than applying a broad mechanical force to the specimen. This makes pressure regulation central to targeted recovery: sufficient suction supports collection, while controlled application helps limit disruption of nearby structures. That balance is especially important when the desired material lies within complex neural tissue.
The fluid-filled pipette provides the immediate pathway for recovering the selected material after microscopic targeting. Its fine tip supports access to individual cells or small regions, while the contained fluid allows negative pressure to act through the pipette. This combination connects precise selection at the specimen with later morphological, molecular, or physiological analysis.
Microscopic guidance allows investigators to identify the material of interest before applying suction and to monitor its position during recovery. That visual control helps distinguish a selected neuron or cellular region from neighboring structures in a complex specimen. As a result, harvesting can remain focused on the intended target rather than treating the surrounding tissue as an undifferentiated sample.
A typical workflow begins by examining the specimen under a microscope and selecting an individual cell or small tissue region. The investigator then positions the fine, fluid-filled pipette at the target, applies controlled negative pressure, and recovers the selected material. The harvested sample can subsequently be directed toward morphological, molecular, or physiological analysis.
A harvested cell or region can support several complementary lines of investigation. Morphological studies can examine cellular form, molecular studies can characterize cell-associated molecular features, and physiological studies can assess functional properties. Because the material is selected at a targeted cellular scale, these analyses can help relate individual-cell characteristics to neuronal diversity or local circuit organization.
The technique is useful when researchers need to connect a precisely selected neural location with downstream analysis. It can support studies of individual neurons, defined cellular regions, local circuit organization, and disease-related changes. Its value comes from combining targeted recovery with assays that reveal morphology, molecular characteristics, or physiology, allowing cellular differences to be examined within complex neural tissue.