Toxicological Applications
Toxicological Applications - Related Videos
08:39
Generation of Electronic Cigarette Aerosol by a Third-Generation Machine-Vaping Device: Application to Toxicological Studies
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Cited by 31 •
2018
Electronic cigarette (e-cig) users are increasing worldwide. Little, however, is known about the health effects induced by inhaled e-cig aerosols. This article describes an e-cig aerosol generation technique suitable for animal exposures and subsequent toxicological studies. Such protocols are required to establish experimentally reproducible and standardized e-cig exposure systems.
11:38
High Content Screening Analysis to Evaluate the Toxicological Effects of Harmful and Potentially Harmful Constituents (HPHC)
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Cited by 23 •
2016
The objective of the study was to assess the biological impact of 15 cigarette smoke constituents using a combination of an impedance-based real time cell analyzer and a high-content screening (HCS)-based platform for toxicological assessment in vitro. This study provides information on effective doses, toxicity and modes of action of the tested compounds.
08:59
An Intestine/Liver Microphysiological System for Drug Pharmacokinetic and Toxicological Assessment
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Cited by 3 •
2020
We exposed a microphysiological system (MPS) with intestine and liver organoids to acetaminophen (APAP). This article describes the methods for organoid production and APAP pharmacokinetic and toxicological property assessments in the MPS. It also describes the tissue functionality analyses necessary to validate the results.
07:28
A Toxicological and Ecotoxicological Assay Based on Mussel (Mytilus galloprovincialis) Hemocytes Motility
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2024
The article proposes a novel in vitro method for the rapid and sensitive assessment of the toxicity and ecotoxicity of pollutants, based on the motility of Mytilus galloprovincialis hemocytes. The method aims to contribute to the development of more ethical and sensitive toxicological and ecotoxicological exposure tests.
09:33
Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors
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Cited by 1 •
2018
This manuscript describes the use of genetically-encoded fluorogenic reporters in an application of live-cell imaging for the examination of xenobiotic-induced oxidative stress. This experimental approach offers unparalleled spatiotemporal resolution, sensitivity, and specificity while avoiding many of the shortcomings of conventional methods used for the detection of toxicological oxidative stress.
FAQs
These variables determine how much of an agent reaches relevant tissues and how long neural systems are challenged. Dose-response analysis compares exposure levels with changes in neuronal signaling, synaptic function, cellular stress, or behavior. Examining duration and route alongside dose helps distinguish transient effects from progressive injury and supports more precise interpretation of neurotoxicity.
Each approach reveals a different level of nervous system disruption. Cellular models can examine signaling, synaptic processes, or stress responses, whereas animal studies and behavioral assessments show how those changes relate to whole-organism function. Using these complementary models strengthens mechanistic testing by connecting cellular effects with neurobehavioral outcomes rather than relying on a single type of evidence.
Biomarkers provide measurable indicators associated with exposure or nervous system injury. In toxicological applications, they can help connect an agent with changes in neuronal signaling, synaptic function, or cellular stress. Their value lies in clarifying mechanisms and supporting exposure assessment, allowing researchers to compare biological responses and identify evidence relevant to neurotoxicity and risk evaluation.
A study generally characterizes the agent and exposure conditions, then examines dose-response relationships using mechanistic tests and appropriate biological models. Researchers may evaluate cellular or synaptic effects before relating them to animal or behavioral findings. Integrating these results helps determine whether observed changes are consistent with nervous system injury and provides evidence for subsequent risk assessment.
They help determine whether a potential or existing medicine produces harmful effects on neuronal signaling, synaptic function, cellular stress, or behavior. Testing across exposure conditions can reveal risks that may not be apparent from a single measurement. This evidence supports safer compound development and ongoing monitoring by linking therapeutic exposure with possible neurotoxic outcomes.
Toxicological applications provide a framework for examining how pollutants and other chemical, biological, or physical agents affect the nervous system. Researchers assess exposure, dose, duration, and route, then compare mechanistic and behavioral outcomes. The resulting evidence can contribute to risk assessment and public health decisions by identifying hazards and clarifying conditions associated with neural injury.