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Measuring Replicative Life Span in the Budding Yeast
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JoVE Journal Biologie
Measuring Replicative Life Span in the Budding Yeast

Measuring Replicative Life Span in the Budding Yeast

DOI:
10.3791/1209-v

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12:41 min

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June 25, 2009

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, ,
1Department of Biochemistry,University of Washington, 2Department of Pathology,University of Washington

Kapitel

  • 00:05Titel
  • 00:26Introduction
  • 00:49Prepare Strains and Plates for Replicative Life Span Analysis
  • 03:35Position Cells for Replicative Life Span Analysis
  • 06:22Obtain Virgin Daughter Cells for Life Span Analysis
  • 08:37Measuring the Replicative Capacity of Individual Cells
  • 11:18Representative Results
  • 12:10Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

In this article we present a general protocol for measuring the replicative life span of yeast mother cells.

Tags

Replicative Life SpanBudding YeastAgingCellular ComponentsOrganellesMortalitySaccharomyces CerevisiaeBiology Of AgingAge-associated PhenotypesLife Span ParadigmsChronological Life SpanQuiescence-like StatePost-mitotic CellsReplicative Life Span AssayDaughter CellsSenescenceMitotically Active CellsLight Microscope

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