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Kapitel
- 00:00Titel
- 00:31Introduction
- 01:05Preparing the pPICZaA Construct
- 02:18Preparation of Electrocompetent Yeast Cells
- 04:04Transformation by Electroporation
- 06:06Protein Expression in Pichia pastoris
- 08:42Results of Recombinant Protein Expression
- 09:02Conclusion
Protokol Metilotropik maya kullanarak protein ekspresyonu açıklar.<em> Pichia pastoris</em>. Electrocompetent maya hücrelerinin hazırlanması, ilgi gen içine vektör dönüşümü<em> S. pastoris</em> Ve maya DNA arıtma de yapılmaktadır. Western blot analizi ve protein saflaştırma bu protein ifade protokolünde son adımları oluşturmak.
Tags
Recombinant ProteinsPichia PastorisProtein ExpressionMethylotrophic YeastExpression SystemPost-translational ModificationsProtein FoldingProteolytic ProcessingDisulfide Bond FormationGlycosylationMethanol MetabolismAlcohol Oxidase PromoterMethanol InductionSecretion Of Recombinant ProteinSignal SequenceHeterologous ProteinProtein PurificationVector
