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In vivo Visualization of Synaptic Vesicles Within Drosophila Larval Segmental Axons
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JoVE Journal Neurowissenschaften
In vivo Visualization of Synaptic Vesicles Within Drosophila Larval Segmental Axons

In vivo Visualization of Synaptic Vesicles Within Drosophila Larval Segmental Axons

DOI:
10.3791/2151-v

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05:58 min

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October 15, 2010

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,
1Department of Biological Sciences,SUNY-University at Buffalo

Kapitel

  • 00:00Titel
  • 00:49Introduction
  • 01:01Preparation for Dissection
  • 01:51Collection of Larvae
  • 02:16Live Dissection of Larvae
  • 04:18Live Imaging of Dissected Larvae
  • 05:00Representative Images
  • 05:37Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

This protocol discusses the live dissection of Drosophila larvae for the purpose of imaging the movement of GFP tagged axonal vesicles on microtubule tracks.

Tags

In Vivo VisualizationSynaptic VesiclesDrosophila Larval Segmental AxonsAxonal TransportNeurological DiseasesDissection ProtocolLive ImagingUAS-GAL4 MethodGFP-tagged APPSynaptotagmin VesiclesNeuronal GAL4 DriversFluorescently Tagged MarkersMitochrondriaMitoTrackerLysosomesLysoTrackerGFP-vesicle MovementParticle Movement

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