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Journal / Immunology and Infection / Hybridization in situ of Salivary Glands,…
JoVE Journal
Immunology and Infection

Dieser Inhalt ist Open Access.

JoVE Journal Immunology and Infection
Hybridization in situ of Salivary Glands, Ovaries, and Embryos of Vector Mosquitoes
Separation of the Cell Envelope for Gram-negative Bacteria into Inner and Outer Membrane Fractions with Technical Adjustments for Acinetobacter baumannii
Journal (Immunology and Infection)
Separation of the Cell Envelope for Gram-negative Bacteria into Inner and Outer Membrane Fractions with Technical Adjustments for Acinetobacter baumannii
Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function
Journal (Immunology and Infection)
Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function
 

Hybridization in situ of Salivary Glands, Ovaries, and Embryos of Vector Mosquitoes

Article DOI: 10.3791/3709-v • 10:58 min • June 28th, 2012
June 28th, 2012 •
1, 1,2
1Department of Molecular Biology and Biochemistry, University of California, Irvine, 2Department of Microbiology and Molecular Genetics, University of California, Irvine

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Summary
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Temporal and spatial gene expression analyses have a crucial role in functional genomics. Whole-mount hybridization in situ is useful for determining the localization of transcripts within tissues and subcellular compartments. Here we outline a hybridization in situ protocol with modifications for specific target tissues in mosquitoes.

Tags

Hybridization In Situ Salivary Glands Ovaries Embryos Vector Mosquitoes Pathogens Transcripts Gene Regulators Mosquito-borne Disease Transmission Egg Development Gene Expression Regulation Spatial Information Accumulation Patterns Female Salivary Glands Ovaries And Embryos Subcellular Compartments Mosquito Reproduction And Development RNA Probe Preparation Dissection Of Soft Tissues Fixation And Hybridization
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