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Simultaneous Electrophysiological Recording and Calcium Imaging of Suprachiasmatic Nucleus Neurons

Article DOI: 10.3791/50794-v • 09:42 min • December 8th, 2013
December 8th, 2013 •
1, 1,2
1Center for Research on Occupational and Environmental Toxicology, Oregon Health & Science University, 2Department of Behavioral Neuroscience, Oregon Health & Science University

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Procedures are described to perform simultaneous recordings of membrane potential or current and changes of intracellular calcium concentration. Suprachiasmatic nucleus neurons are filled with the calcium indicator bis-fura-2 using a patch clamp electrode in the whole cell patch clamp configuration.

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Simultaneous Electrophysiological Recording Calcium Imaging Suprachiasmatic Nucleus Neurons Membrane Potential Current Intracellular Calcium Concentration Environmental Conditions Light-dark Cycle Neuronal Activity Neural Network Activity Circadian Clock Genes Excitatory Synaptic Transmission Postsynaptic Ca2+ Concentration Signaling Pathways Rhythmic Expression Hypothalamic Slices Patch Clamp Microelectrodes Calcium Indicator Bis-fura-2 Seal Formation Membrane Rupture Calcium Probe Diffusion Soma And Dendrites Quantitative Ratiometric Measurements Synaptic Activity Action Potential Firing
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