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Immunology and Infection

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Attivazione e misurazione dei NLRP3 inflammasome all'attività utilizzando IL-1β in cellule dendritiche umane derivate da monociti

Article DOI: 10.3791/51284-v • 09:04 min • May 22nd, 2014
May 22nd, 2014 •
1, 2, 3
1Department of Pathology, New York University School of Medicine, 2Division of Infectious Diseases, Department of Medicine, Mount Sinai Medical Center, 3Division of Hematology and Oncology, Hess Center for Science and Medicine, Mount Sinai Medical Center

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Le cellule dendritiche (DC) secernono IL-1β in risposta al riconoscimento di TLR8 purinico sintetico, R848, seguita dall'attivazione inflammasome NLRP3 con nigericin, dunque, IL-1β può essere utilizzata per misurare l'attività inflammasome NLRP3. Intracellulare colorazione citochina, immunoblotting, ELISA e sono utilizzati per misurare con precisione NLRP3 inflammasome adescamento e l'attivazione tramite l'espressione di IL-1β.

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Immunologia NLRP3 inflammasome IL-1beta interleuchina-1 beta dendritiche cellule Nigericina Toll-like receptor 8 TLR8 R848 derivate da monociti cellule dendritiche
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