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Kapitel
- 00:05Titel
- 01:26Tissue Preparation
- 02:44Mounting and Flourescence Imaging
- 04:27Image Processing – Automatic Image Alignment
- 05:32Image Processing – Composite Sharpening
- 06:52Image Processing – Adjustments and Animation
- 08:02Results: Complex Developmental Gradients Pattern the Drosophila Eye
- 09:32Conclusion
This protocol presents an efficient method for imaging the live Drosophila pupal eye neuroepithelium. This method compensates for tissue movement and uneven topology, enhances visualization of cell boundaries through the use of multiple GFP-tagged junction proteins, and uses an easily-assembled imaging rig.
