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Исследуя одну молекулу адгезии методом атомно-силовой микроскопии
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Ingenieurwesen
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JoVE Journal Ingenieurwesen
Investigating Single Molecule Adhesion by Atomic Force Spectroscopy
DOI:

09:48 min

February 27, 2015

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Kapitel

  • 00:05Titel
  • 01:25Tip Functionalization
  • 06:01Data Acquisition
  • 07:50Results: Single Molecule Adhesion of Polytyrosine on a Methyl-self-assembled Monolayer
  • 09:19Conclusion

Summary

Automatische Übersetzung

A protocol to couple a large variety of single molecules covalently onto an AFM tip is presented. Procedures and examples to determine the adhesion force and free energy of these molecules on solid supports and bio-interfaces are provided.

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