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封装产热前脂肪细胞移植到脂肪组织仓库
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Medizin
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JoVE Journal Medizin
Encapsulation Thermogenic Preadipocytes for Transplantation into Adipose Tissue Depots
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封装产热前脂肪细胞移植到脂肪组织仓库

DOI:
10.3791/52806-v

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08:30 min

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June 02, 2015

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, , , ,
1Department of Human Sciences,The Ohio State University, 2Department of Minimally Invasive Surgery,The First Affiliated Hospital of Soochow University, 3NSF Nanoscale Science and Engineering Center for Affordable Nanoengineering of Polymeric Biomedical Devices,The Ohio State University

Kapitel

  • 00:05Titel
  • 01:30Alginate Microbeads Preparation
  • 03:55Coating Microbeads with Poly-L-lysine (PLL) and Removing the Alginate Core
  • 05:45In Vitro Applications to Study Xenograft and Host Cell Interactions or Kinetics of Metabolite Influx/Efflux Between Cells
  • 06:34Results: Successful Encapsulation and Transplantation of Preadipocytes Into Fatty Tissue of Mice
  • 07:59Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Here, we present a protocol for encapsulation of catabolic cells, which consume lipids for heat production in intra-abdominal adipose tissue and increase energy dissipation in obese mice.

Tags

Cell EncapsulationPreadipocytesThermogenicTransplantationSemi-permeable MembraneImmune SystemBioactive CompoundsInsulinHormonesCytokinesThermogenesisObesityType 2 DiabetesDetoxificationAlcoholsToxic MetabolitesEnvironmental Pollutants

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