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Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile
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JoVE Journal Medizin
Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile

Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile

DOI:
10.3791/54036-v

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06:59 min

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June 13, 2016

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, , , ,
1Division of Gastroenterology and Hepatology, Department of Medicine,Johns Hopkins University School of Medicine, 2Department of Surgery,Tohoku University

Kapitel

  • 00:05Titel
  • 00:38Collection of Human Bile
  • 02:14Exosome Isolation from Human Bile and Transmission Electron Microscopy
  • 04:16Results: Characterization of MicroRNA-containing Exosomes from Human Bile
  • 05:22Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Bile fluid is a valuable source of extracellular vesicles/exosomes that contain potentially important biomarkers. This protocol represents a robust method to isolate exosomes from human bile for further analyses including miRNA profiling.

Tags

Keyword Extraction:MicroRNAExosomesBileERCPEndoscopic Retrograde CholangiopancreatographyCholangiocarcinomasBiliary DiseasesUltracentrifugation0.2-micrometer FilterCentrifugationBile Marker

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