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Stripe Assay по изучению притягательные или отталкивающие активность белка подложки с использованием диссоциированных нейронах гиппокампа
JoVE Journal
Neurowissenschaften
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JoVE Journal Neurowissenschaften
Stripe Assay to Study the Attractive or Repulsive Activity of a Protein Substrate Using Dissociated Hippocampal Neurons
DOI:

08:11 min

June 19, 2016

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Kapitel

  • 00:05Titel
  • 00:46Preparing the Matrices
  • 01:43Stripe Generation and Laminin Coating for Neuron Cultures
  • 03:59Culturing Hippocampal Neurons fom E15.5 Mouse Embryos
  • 06:36Results: Hippocampal Neuron Growth over FLRT2 Coated Substrate
  • 07:36Conclusion

Summary

Automatische Übersetzung

Axon guidance molecules regulate neuronal migration and targeted growth-cone navigation. We present a powerful method, the stripe assay, to assess the ability of guidance molecules to attract or repulse neurons. In this protocol, we demonstrate the stripe assay by showing FLRT2's ability to repel cultured hippocampal neurons.

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