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Kapitel
- 00:00Titel
- 01:54Seeding of N9 Microglia in Inserts
- 03:47Activation of N9 Microglia With Lipopolysaccharide
- 05:02Co-Cultivation of Neuronal PC12 Cells With N9 Microglia
- 07:31Results: Lipopolysaccharide-activated N9 Microglia Secrete Cytokines That Are Toxic to Neuronal PC12 Cells
- 09:48Conclusion
In multicellular organisms, secreted soluble factors elicit responses from different cell types as a result of paracrine signaling. Insert co-culture systems offer a simple way to assess the changes mediated by secreted soluble factors in the absence of cell-cell contact.
