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Kapitel
- 00:05Titel
- 01:07Rat Heart Isolation and Cannulation
- 04:51Measurement of Cardiac Function and Sample Collection
- 07:17Determination of Myocardial Glucose Oxidation Rate
- 08:48Determination of Myocardial Oleate Oxidation Rates
- 10:14Results: Measurements from Two Stressed Hearts
- 11:40Conclusion
The following protocol describes the preparation and utilization of buffers for the quantitative measurement of rates of glucose and fatty acid oxidation in the isolated working rat heart. The methods used for sample analysis and data interpretation are also discussed.
