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Journal / Developmental Biology / Organ Culture and Whole Mount Immunofluorescence…
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Developmental Biology

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JoVE Journal Developmental Biology
Organ Culture and Whole Mount Immunofluorescence Staining of Mouse Wolffian Ducts
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Organ Culture and Whole Mount Immunofluorescence Staining of Mouse Wolffian Ducts

Article DOI: 10.3791/55134-v • 09:16 min • January 13th, 2017
January 13th, 2017 •
1, 1
1Gynaecology Oncology Group, School of Biomedical Sciences and Pharmacy, University of Newcastle

Kapitel

Summary
Automatic Translation

We present a method for isolation and culture of the mouse Wolffian duct (WD). We also demonstrate a detailed procedure for whole mount immunostaining of cultured/freshly isolated WDs with fluorescently tagged antibodies. Together, these techniques enable the study of WD development, coiling, and differentiation.

Tags

Organ Culture Whole Mount Immunofluorescence Staining Mouse Wolffian Ducts Developmental Biology Organ Shape Organ Structure Organ Function In Vivo Systems Cell Culture Studies Niche Factors Post-conception Pregnant Mouse Surgical Procedure Uterus HBSS Solution
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