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Bioengineering

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JoVE Journal Bioengineering
利用 CRISPR-Cas9 系统高效生成和编辑人胰细胞无饲养干细胞
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利用 CRISPR-Cas9 系统高效生成和编辑人胰细胞无饲养干细胞

Article DOI: 10.3791/56260-v • 09:16 min • November 8th, 2017
November 8th, 2017 •
1,2, 3, 1,2,4
1Department of Animal and Avian Sciences, University of Maryland, 2Animal Bioscience and Biotechnology Laboratory, ARS, USDA, 3NIH Stem Cell Unit, Bethesda, National Institutes of Health, 4RenOVAte Biosciences Inc

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Summary
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本协议详细描述了在无饲养条件下从人类胰腺细胞中生成无足迹诱导多能干细胞 (干细胞), 其次是使用 CRISPR/Cas9 均一进行编辑和修饰单细胞克隆。

Tags

生物工程 问题 129 诱导多能干细胞 (干细胞) 重编程 仙台病毒 CRISPR/Cas9 无足迹干细胞 nucleofection
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