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Journal / Bioengineering / 使用非整合方法分离成人人体皮肤纤维细胞与诱导多能干细胞的生成…
JoVE Journal
Bioengineering

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JoVE Journal Bioengineering
使用非整合方法分离成人人体皮肤纤维细胞与诱导多能干细胞的生成
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使用非整合方法分离成人人体皮肤纤维细胞与诱导多能干细胞的生成

Article DOI: 10.3791/60629-v • 10:52 min • January 19th, 2020
January 19th, 2020 •
*1, *1, 1, 1, 1, 1, 1, 1
1Department of Public Health, University of Naples Federico II
* These authors contributed equally

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Summary
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细胞重新编程需要引入关键基因，这些基因调节和维持多能细胞状态。所述方案使诱导多能干细胞（iPSCs）菌落的形成来自人类皮肤成纤维细胞，无需病毒/整合方法，但使用非修饰RNA（NM-RNA）结合免疫逃逸因子减少细胞防御机制。

Tags

生物工程，第155期，诱导多能干细胞（iPSC），细胞再编程，皮肤成纤维细胞，非改性RNA，NM-RNA，无一体化方法，细胞培养，人类，再生医学
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