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Kapitel
- 00:04Titel
- 00:33Prepare Infiltration Suspension
- 02:11Perform Vacuum Infiltration
- 03:56Growth and Harvest of the Infiltrated Leaves and Pressurized Solvent Extraction
- 05:38Basic Ion Exchange Resin Treatment for Removal of Chlorophylls
- 06:58Results: Infiltration Coverage and Isolated Triterpene Product
- 07:49Conclusion
Midlertidig heterologous uttrykk for biosyntetiske enzymer i Nicotiana benthamiana blader kan avlede endogene forsyninger av 2,3-oxidosqualene mot produksjon av nye høy verdi triterpene produkter. Her er beskrevet en detaljert protokoll for rask (5 dager) skytevåpen skala produksjon av triterpenes og analogs utnytte denne kraftig plante-baserte plattformen.
Tags
Transient ExpressionNicotiana BenthamianaTriterpene ProductionPreparative ScaleAgrobacterium TumefaciensCo-infiltrationVacuum InfiltrationMMA BufferOD 600Preparative-scale AccessStructural CharacterizationMedicinally Relevant Biological ActivitySelective LB AgarSelective LB MediaCentrifugationResuspensionInfiltration Suspension
