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JoVE Journal
Biochemistry

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Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale
 

Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale

Article doi: 10.3791/58169
August 16th, 2018

Summary August 16th, 2018

Transient heterologous expression of biosynthetic enzymes in Nicotiana benthamiana leaves can divert endogenous supplies of 2,3-oxidosqualene towards the production of new high-value triterpene products. Herein is described a detailed protocol for rapid (5 days) preparative-scale production of triterpenes and analogs utilizing this powerful plant-based platform.

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