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Isolation and Characterization of Mouse Primary Liver Sinusoidal Endothelial Cells
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December 16, 2021
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Here we outline and demonstrate a protocol for primary mouse liver sinusoidal endothelial cell (LSEC) isolation. The protocol is based on liver collagenase perfusion, nonparenchymal cell purification by low-speed centrifugation, and CD146 magnetic bead selection. We also phenotype and characterize these isolated LSECs using flow cytometry and scanning electron microscopy.
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Guo, Q., Furuta, K., Aly, A., Ibrahim, S. H. Isolation and Characterization of Mouse Primary Liver Sinusoidal Endothelial Cells. J. Vis. Exp. (178), e63062, doi:10.3791/63062 (2021).
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