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Journal / Bioengineering / In vivo Billeddannelse af biologisk væv med…
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Bioengineering

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In vivo Billeddannelse af biologisk væv med kombineret to-fotonfluorescens og stimuleret Raman-spredningsmikroskopi
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In vivo Billeddannelse af biologisk væv med kombineret to-fotonfluorescens og stimuleret Raman-spredningsmikroskopi

Article DOI: 10.3791/63411-v • 09:06 min • December 20th, 2021
December 20th, 2021 •
1, 1, 1,2,3,4, 5
1Department of Electronic and Computer Engineering, The Hong Kong University of Science and Technology, 2State Key Laboratory of Molecular Neuroscience, The Hong Kong University of Science and Technology, 3Center of Systems Biology and Human Health, The Hong Kong University of Science and Technology, 4Molecular Neuroscience Center, The Hong Kong University of Science and Technology, 5Department of Biology, School of Life Sciences, Southern University of Science and Technology

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Stimuleret Raman scattering (SRS) mikroskopi tillader etiketfri billeddannelse af biomolekyler baseret på deres iboende vibration af specifikke kemiske bindinger. I denne protokol beskrives den instrumentelle opsætning af et integreret SRS og to-fotonfluorescensmikroskop for at visualisere cellulære strukturer i rygmarven hos levende mus.

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