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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolating Bronchial Epithelial Cells from Resected Lung Tissue for Biobanking and Establishing Well-Differentiated Air-Liquid Interface Cultures
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Isolating Bronchial Epithelial Cells from Resected Lung Tissue for Biobanking and Establishing Well-Differentiated Air-Liquid Interface Cultures

Article DOI: 10.3791/65102-v • 08:42 min • May 26th, 2023
May 26th, 2023 •
1, 1, 1
1PulmoScience Laboratory, Department of Pulmonology, Leiden University Medical Center

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Summary
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Presented here is a reproducible, affordable, and robust method for the isolation and expansion of primary bronchial epithelial cells for long-term biobanking and the generation of differentiated epithelial cells by culture at the air-liquid interface.

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Isolating Bronchial Epithelial Cells Resected Lung Tissue Biobanking Well-differentiated Air-liquid Interface Cultures Research Questions Function Of Airway Epithelial Cells Health And Disease Advantages Of The Technique Cell Layer Representation Mucus Production Ciliary Activity Disease-related Insults Therapies Rinsing Bronchial Ring Connective Tissue Removal Blood Remnants Removal Incubation With Protease 14 Solution Scraping Inside The Ring Obtaining Cell Solution Centrifugation
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