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Basic Methods in Cellular and Molecular Biology
PCR Steps and Reaction Setup
Description
PCR uses thermocycling to make many copies of DNA. Thermocycling means repeating cycles of temperature changes at fixed time intervals. With a thermostable DNA polymerase and DNA building blocks called dNTPs, the reaction can produce numerous copies from a small DNA sample.
The PCR cycle has thre...
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Transcript
La reacción en cadena polimerasa o PCR es un método ampliamente usado para amplificar fragmentos de ADN. PCR utiliza termociclaje rápido, que es el repetido calentamiento y enfriamiento de la reacción a través de tres temperaturas distintas, llamada desnaturalización, recocido y extensión o alargamiento.
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