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Basic Methods in Cellular and Molecular Biology
Making Electrocompetent Bacterial Cells
Description
Bacterial transformation can be done by making electrocompetent cells. In this process, foreign DNA enters bacterial cells after an electrical field creates small pores in the cell wall. The cells that can take up DNA from the environment are called competent cells. In the laboratory, scientists can produce electrocompetent ...
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Transcript
Transformación bacteriana es un proceso natural, en el cual las bacterias ingieren ADN extraño y luego amplificarán o clonación. En el laboratorio, este proceso puede ser inducido artificialmente, mediante el uso de pulsos de campo eléctrico de alto voltaje para crear poros en la membrana celular bacteriana, a tr...
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