Transgenic Rodent Cells
Transgenic Rodent Cells - Related Videos
14:45
Transgenic Rodent Assay for Quantifying Male Germ Cell Mutant Frequency
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Cited by 28 •
2014
De novo mutations in the male germline may contribute to adverse health outcomes in subsequent generations. Here we describe a protocol for the use of a transgenic rodent model for quantifying mutations in male germ cells induced by environmental agents.
02:16
Differentiating Non-Transgenic and Transgenic Human Pluripotent Stem Cells into Neural Progenitor Cells
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2025
This video demonstrates the generation of neural progenitor cells from non-transgenic and transgenic human pluripotent stem cells (hPSCs). hPSCs are differentiated into neural progenitor cells using a neuronal differentiation medium. While differentiation is induced in non-transgenic cells using small molecules, the transgenic hPSCs are differentiated by adding an antibiotic that induces the promoter for a neuronal differentiation-specific transcription factor.
07:59
Tracking Cells in GFP-transgenic Zebrafish Using the Photoconvertible PSmOrange System
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Cited by 2 •
2016
We established the photoconvertible PSmOrange system as a powerful, straight-forward and cost inexpensive tool for in vivo cell tracking in GFP transgenic backgrounds. This protocol describes its application in the zebrafish model system.
05:15
Viral Transgene Expression in Rodent Hearts and the Assessment of Cardiac Arrhythmia Risk
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Cited by 2 •
2022
The present protocol describes methods for transgene expression in rat and mouse hearts by direct intramyocardial injection of the virus under echocardiography guidance. Methods for the assessment of the susceptibility of hearts to ventricular arrhythmias by the programmed electrical stimulation of isolated, Langendorff-perfused hearts are also explained here.
06:04
Performing Subretinal Injections in Rodents to Deliver Retinal Pigment Epithelium Cells in Suspension
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Cited by 32 •
2015
Here we present a community accepted protocol in multimedia format for subretinally injecting a bolus of RPE cells in rats and mice. This approach can be used for determining rescue potentials, safety profiles, and survival capacities of grafted RPE cells upon implantation in animal models of retinal degeneration.
FAQs
The promoter or regulatory system linked to the introduced DNA sequence determines how the selected protein is produced. This allows researchers to associate transgene activity with a defined experimental design rather than relying on unregulated expression. In immunology, that control can help examine immune signaling or receptor activity and relate protein production to a specific host response.
Integration places the introduced DNA sequence within the cell’s genome, allowing the transgene to direct production of the selected protein as part of the engineered cellular system. This provides a stable genetic context for investigating how that protein affects cellular behavior, disease mechanisms, or immune responses, rather than examining the gene sequence in isolation.
Reporter-based systems can provide an experimental readout associated with the engineered genetic system, while conditional systems allow researchers to design gene activity around defined experimental conditions. These approaches are useful when the goal is to follow immune signaling, receptor activity, pathogen entry, or host responses with greater experimental control than a single constitutively observed outcome.
Researchers introduce the selected DNA sequence into embryonic cells or early embryos, after which the sequence becomes integrated into the genome and can direct production of the chosen protein. The resulting engineered cells provide a controlled model for subsequent studies of gene function, disease processes, immune activity, or interactions relevant to infection research.
These cells are useful when investigators need to examine immune signaling, receptor activity, pathogen entry, or host responses under controlled genetic conditions. Their defined transgene provides a way to connect a selected protein or genetic activity with an experimental response, supporting mechanistic studies of how immune and infection-related processes contribute to disease.
Transgenic rodent cells can support investigation of disease mechanisms, evaluation of vaccines and therapeutics, and development of conditional or reporter-based experimental systems. In practice, they help connect engineered gene activity with measurable questions about host responses or infection-related biology, making them relevant both to fundamental immunology and to studies assessing potential interventions.