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A Simplified System for Evaluating Cell Mechanosensing and Durotaxis In Vitro
JoVE Revista
Biología
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JoVE Revista Biología
A Simplified System for Evaluating Cell Mechanosensing and Durotaxis In Vitro
DOI:

09:50 min

August 27, 2015

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Capítulos

  • 00:05Título
  • 01:33Preparation of Durotaxis Chambers
  • 03:56Cell Plating
  • 05:33Live-cell Imaging
  • 06:48Data Analysis
  • 08:17Results: The Focal Adhesion Protein, cdGAP, is Required for Durotaxis
  • 09:24Conclusion

Summary

Traducción Automática

Many mammalian cells preferentially migrate towards a more rigid matrix or substrate through durotaxis. The goal of this protocol is to provide a simple in vitro system that can be used to study and manipulate cell durotaxis behaviors by incorporating polydimethylsiloxane (PDMS) substrates of defined rigidity, interfacing with glass coverslips.

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