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A Simplified System for Evaluating Cell Mechanosensing and Durotaxis In Vitro

Article DOI: 10.3791/52949
August 27th, 2015 •
1, 1, 1
1Department of Cell & Developmental Biology, SUNY Upstate Medical University

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August 27th, 2015

Many mammalian cells preferentially migrate towards a more rigid matrix or substrate through durotaxis. The goal of this protocol is to provide a simple in vitro system that can be used to study and manipulate cell durotaxis behaviors by incorporating polydimethylsiloxane (PDMS) substrates of defined rigidity, interfacing with glass coverslips.

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