Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.
Capítulos
- 00:05Título
- 01:33Perfusion and Cell Collection
- 04:03Kupffer Cell Purification
- 06:18Modified Lactate Dehydrogenase Assay
- 07:34Results: Nanoparticle Toxicity Testing on Purified Kupffer Cells
- 09:11Conclusion
Liver macrophages, named Kupffer cells, are responsible for the capture of circulating nanoparticles. We describe here a method, of high cell purity and yield, for Kupffer cell isolation. The modified LDH assay is used here to measure the toxicity induced by carbon nanotubes in Kupffer cells.
