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Journal / Biochemistry / 使用SPHIRE的电子冷冻显微镜图像的高分辨率单粒子分析…
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Biochemistry

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JoVE Journal Biochemistry
使用SPHIRE的电子冷冻显微镜图像的高分辨率单粒子分析
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使用SPHIRE的电子冷冻显微镜图像的高分辨率单粒子分析

Article DOI: 10.3791/55448-v • 13:28 min • May 16th, 2017
May 16th, 2017 •
1, 1, 1, 1, 2, 2, 2, 1, 1
1Department of Structural Biochemistry, Max Planck Institute of Molecular Physiology, 2Department of Biochemistry and Molecular Biology, The University of Texas Medical School at Houston

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本文介绍了使用软件套件SPHIRE处理低温EM图像的协议。目前的协议可以应用于几乎所有瞄准近原子分辨率的单粒子EM项目。

Tags

生物化学，第123期，结构生物学，电子显微镜，电子冷冻显微镜，低温EM，TEM，单粒子图像处理，软件开发，单粒子分析，Tc毒素
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