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Forbedring af anvendelsen af høj molekylvægt Biotinylated Dextran Amin for Thalamocortical projektion sporing i rotter

Article DOI: 10.3791/55938-v • 06:39 min • April 12th, 2018
April 12th, 2018 •
*1, *1, 1, 1, 1, 1
1Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences
* These authors contributed equally

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Vi præsenterer her, en raffineret protokol for at effektivt afsløre biotinylated dextran Amin (BDA) mærkning med en fluorescerende farvning metode gennem en gensidig neurale vej. Det er egnet til at analysere fine strukturen af BDA mærkning og adskille det fra andre neurale elementer under en Konfokal laser scanning mikroskop.

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Neurovidenskab spørgsmål 134 Biotinylated dextran Amin tracer neurale mærkning axon neuron somatosensoriske cortex
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