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Biochemistry

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JoVE Journal Biochemistry
Indfange interaktion kinetik af en Ion-kanal Protein med små molekyler af Bio-lag interferometri Assay
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Indfange interaktion kinetik af en Ion-kanal Protein med små molekyler af Bio-lag interferometri Assay

Article DOI: 10.3791/56846-v • 10:41 min • March 7th, 2018
March 7th, 2018 •
1, 2, 1, 3
1Department of General Surgery, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, 2Key Laboratory of Systems Biomedicine (Ministry of Education), Institute of Systems Biomedicine, Shanghai Jiao Tong University, 3Hongqiao International Institute of Medicine, Tongren Hospital, Shanghai Jiao Tong University School of Medicine

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Protokol her beskriver renset hEAG1 ion-kanal protein interaktioner med lille molekyle lipid ligand phosphatidylinositol 4, 5-bisfosfat (PIP2). Målingen viser, at BLI kunne være en potentiel metode til romanen små-molekyle ion-kanal ligand screening.

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Biokemi sag 133 Bio-lag interferometri (BLI) ion-kanal protein lille molekyle protein affinitet rensning pattedyr stabil ekspressionssystem menneskelige EAG1 (hEAG1) kanal drug discovery terapeutiske mål ligand screening
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