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Biochemistry

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JoVE Journal Biochemistry
Fange samhandling Kinetics en Ion kanal protein med små molekyler av Bio-lags Interferometry analysen
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Fange samhandling Kinetics en Ion kanal protein med små molekyler av Bio-lags Interferometry analysen

Article DOI: 10.3791/56846-v • 10:41 min • March 7th, 2018
March 7th, 2018 •
1, 2, 1, 3
1Department of General Surgery, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, 2Key Laboratory of Systems Biomedicine (Ministry of Education), Institute of Systems Biomedicine, Shanghai Jiao Tong University, 3Hongqiao International Institute of Medicine, Tongren Hospital, Shanghai Jiao Tong University School of Medicine

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Protokollen her beskriver samspillet renset hEAG1 ion kanal protein med små molekyl lipid ligand phosphatidylinositol 4, 5-bisphosphate (PIP2). Målingen viser at BLI kan være en potensiell metode for romanen lite molekyl ion kanal ligand screening.

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Biokjemi problemet 133 Bio-lags Interferometry (BLI) ion kanal protein små molekyl protein affinitet rensing pattedyr stabil uttrykk system menneskelig EAG1 (hEAG1) kanal medisiner terapeutiske mål ligand screening
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